Project

Part:BBa_K1462410

Designed by: Ye Peng   Group: iGEM14_SCUT   (2014-10-10)


pTEF2+GroES+tADH1+pTEF2+GroEL+tADH1+pGAL1+PRK+GBD-ligand+tADH1+pTDH3+RuBisCo+SH3-ligand+tADH1+pTDH3+

promoter TEF2 + chaperon GroES + terminator ADH1 + promoter TEF2 + chaperon GroEL + terminator ADH1 + promoter GAL1+PRK+ligand of the scaffold GBD+ terminator ADH1 promoter TDH3+RuBisCo+ligand of the scaffold SH3+ terminator ADH1 promoter TDH3+CA+ligand of the scaffold PDZ+ terminator ADH1


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4138
    Illegal BglII site found at 8442
    Illegal BglII site found at 9468
    Illegal BamHI site found at 10286
    Illegal XhoI site found at 10267
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3639
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None